---
title: "How to Create a Peptide SAR Report"
slug: "how-to-create-peptide-sar-report"
status: "update"
updated: 2026-10-14T09:22:34Z
published: 2026-10-14T09:22:34Z
canonical: "docs.discngine.com/how-to-create-peptide-sar-report"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# How to Create a Peptide SAR Report

A **SAR Report** is a workspace that holds one or more **SAR Slides**. This guide creates a peptide report and its first slide (a viewer built around a reference peptide and an alignment), then shows how to add more slides.

## Step 1: Open the Create SAR Report dialog

From the **Home** page click the **Create SAR Report** card. In the dialog, pick a peptide dataset from the sidebar (peptide datasets are tagged **Peptide**). When a peptide dataset is selected, the report type is set to peptide automatically and the configuration pane appears on the right.

![The Create new SAR Report dialog with the Glucagon peptide dataset selected, showing the Reference Peptide selector and the Alignment section](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Documentation/create-sar-report-peptide.png?sv=2026-02-06&spr=https&st=2026-10-27T12%3A05%3A54Z&se=2026-10-27T13%3A05%3A54Z&sr=c&sp=r&sig=YSSt9oK6itj2zG%2FVOd%2FFb7ptItdrE4zyp6zRU3rjD5I%3D)

## Step 2: Choose the reference peptide

Under **Reference Peptide**, select the peptide to use as the basis for the analysis (*"Select the reference peptide to use as the basis for the SAR analysis."*). If the dataset has a default reference peptide, it is pre-selected — you can change it here.

## Step 3: Pick the alignment

Every peptide slide needs an **alignment** that lines the peptides up position-by-position. The good news is that two are already there: importing a peptide dataset computes a **Center-star alignment** and a **PepSeA alignment** automatically, listed under **Available Alignments** with a **Center-star** or **PepSeA** badge. The newest completed one is preselected — keep it, or pick the other, and move on. Only a completed alignment can be used: one that still reads **In progress** becomes selectable once it finishes.

If you would rather use your own, or compute a fresh one, click **New Alignment** and pick **Import from a file**, **Align with center-star** or **Align with PepSeA**.

![The New Alignment button expanded, showing the Import from a file, Align with center-star and Align with PepSeA entries](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Documentation/new-alignment-menu.png?sv=2026-02-06&spr=https&st=2026-10-27T12%3A05%3A54Z&se=2026-10-27T13%3A05%3A54Z&sr=c&sp=r&sig=YSSt9oK6itj2zG%2FVOd%2FFb7ptItdrE4zyp6zRU3rjD5I%3D)

An amber icon on an alignment row means the imported file didn't fully agree with the dataset; the pencil icon opens the alignment editor, where sequence mismatches can be repaired.

Alignments belong to the dataset, so every alignment you add here stays available to future reports and slides.

Full details

The file format, the Excel backbone/branch sheet steps, what each import warning means, and how to fix a sequence mismatch are covered in [How to Align Peptides](/public/docs/how-to-align-peptides).

## Step 4: Generate the first slide

With a reference peptide and an alignment selected, click **Generate SAR Slide**. The report opens with its first slide in the [peptide viewer](/public/docs/how-to-explore-peptide-viewer).

## Step 5: Add more slides (optional)

Inside an open report, use the **Add SAR Slide** button in the tab bar to create another slide in a new tab — for example with a different reference peptide or a different alignment — so you can compare views within the same report. Existing slides can also be duplicated.

![The peptide viewer tab bar showing the current slide tab and the Add SAR Slide button](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Documentation/viewer-branched-overview.png?sv=2026-02-06&spr=https&st=2026-10-27T12%3A05%3A54Z&se=2026-10-27T13%3A05%3A54Z&sr=c&sp=r&sig=YSSt9oK6itj2zG%2FVOd%2FFb7ptItdrE4zyp6zRU3rjD5I%3D)

## Next steps

- [How to Align Peptides](/public/docs/how-to-align-peptides)
- [How to Explore the Peptide Viewer](/public/docs/how-to-explore-peptide-viewer)
- [How to Analyze Hotspots and Synergies](/public/docs/how-to-analyze-hotspots-and-synergies)
- [How to Cluster Peptides](/public/docs/how-to-cluster-peptides)