---
title: "Plate Serial Dilution Overview"
slug: "plate-serial-dilution-overview"
updated: 2026-09-09T14:03:10Z
published: 2026-09-09T14:03:10Z
canonical: "docs.discngine.com/plate-serial-dilution-overview"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Plate Serial Dilution Overview

The `Plate Serial Dilution` workflow in Sample enables users to perform a series of dilutions across a plate, following either a predefined layout or a sequence of user-defined tasks. Serial dilution is a fundamental laboratory technique used to create a range of concentrations from a single stock solution, supporting applications such as dose-response assays, standard curve generation, and compound screening.

This workflow allows users to perform dilutions from well to well across a plate. Two modes of sample dilution are available:

- **Dilution by row** – the concentration gradient is created within the same row
- **Dilution by column** – the concentration gradient is created within the same column

With this workflow, you can:

- Automate the process of creating dilution series across wells
- Customize dilution layouts and steps to fit specific experimental requirements
- Use predefined dose layouts for standardized dilutions
- Create custom manual dilution tasks for specific needs

![Plate Serial Dilution example](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Setting/sample-plate-serial-dilution-example.png?sv=2026-02-06&spr=https&st=2026-11-11T13%3A43%3A55Z&se=2026-11-11T14%3A43%3A55Z&sr=c&sp=r&sig=bvooyISVa2jfC7m9L47fouKWsFddalJwVOyXeC%2Fn20M%3D)

## Related Tutorials

- [Tutorial - Dose Layout](/public/docs/tutorial-serial-dilution-with-a-dose-layout) - Step-by-step guide to create serial dilutions using predefined dose layouts
- [Tutorial - Manual Dilution](/public/docs/tutorial-manual-serial-dilution) - Step-by-step guide to create serial dilutions with custom manual tasks