---
title: "Tutorial - Plate Replication"
slug: "tutorial-plate-replication"
updated: 2026-09-09T14:03:05Z
published: 2026-09-09T14:03:05Z
canonical: "docs.discngine.com/tutorial-plate-replication"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Tutorial - Plate Replication

## Introduction

In this tutorial, you will make two copies of five 384-well plates (100 µL – 10 mM). The created plates will have a volume of 30 µL and will be diluted by a factor of 10 (resulting in 30 µL – 1 mM).

## Prerequisite

              Required permissions and setup

              

**Sample Processing** role is required to access this workflow.

**Required system configuration:**

- Container Types: `Greiner PP384V`,
- Container Usage `Inventory`,
- Solvent `DMSO`,
- Worklist Generator and Parser: `Standard (Container) (;)`

These should exist by default in the Sample application.

### Items

Containers and locations created from Discngine Sample’s automation will be used:

- Five 384-well plates (100 µL – 10 mM). **Layout with columns 1 and 24 as empty.**
- Location `Paris`  

Automation is running during installation if “example_dataset” is set to true in the configuration file.

## Workflow

### Job Initialization

[Create a new job](/public/docs/jobs-overview#create-a-job) and select the `Plate Replication` workflow.

### Requesting

- Select **Plate** as Source Type
- Choose Target Plate Barcode as **Generate New Barcode**
- Select **Greiner PP384V** as the Target Plate Type
- Specify Request from **Text**
- Copy/paste the plate names in the text area: **PTEST006:PTEST007:PTEST008:PTEST009:PTEST010**  

Plate names can be separated by colons or line breaks.
- Click `Add IDs` to confirm. The plates are listed in the added requests.
- Click `Next`

### Task Requesting

- Choose Source Selection Mode = **All sources**
- Target Usage = **Inventory**
- Select Number of Copy = **2**
- Target Volume = **30 μl**
- Dilution = **Target**
- Dilution Mode = **Dilution Factor**
- Dilution Factor = **10**
- Buffer (Solvent) = **DMSO**
- Offset Volume = **0%**
- Offset Solvent = **0%**
- Click `Add Task`. Two tasks are added (one for each copy).
- Click `Next`

### Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source plates (information in the `Sourced` column).

- Click `Next`

### Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

- Select the **Standard (Container) (;)** equipment and set File Order to **By Request**
- Click `Generate worklist`

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing the information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

- Click `Copy Worklist`
- Click `Next` to propagate those changes in the system.

The tasks are processed in the Sample application.

### Storing

The [Storing step](/public/docs/jobs-overview#storing) allows you to assign a location to the items involved in the job. First, for the output plates (plates created during the job).

- Click `Assign Location to Selected`
- Select **Paris** and click `Select` to confirm your choice.
- Click `Next` to continue to the input plates. Here, we won’t change the input plates location.
- Click `Next`

You can increment the Thawing Cycle count of the input plates if necessary.

### Pipe & Close

The [Pipe & Close step](/public/docs/jobs-overview#pipe-close) provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one.

For this tutorial, click the `Close & Quit` button to finish the job.