---
title: "Tutorial - Plate Solubilization"
slug: "tutorial-plate-solubilization"
updated: 2026-09-09T14:03:30Z
published: 2026-09-09T14:03:30Z
canonical: "docs.discngine.com/tutorial-plate-solubilization"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Tutorial - Plate Solubilization

## Introduction

In this tutorial, you will add solvent to solid batches in a plate to convert them to liquid state.

## Prerequisites

              Required permissions and setup

              

**Sample Processing** role is required to access this workflow.

**Required system configuration:**

- Container Types: `Greiner PP384V`
- Container Usage: `Inventory`
- Solvent: `DMSO`
- Worklist Generator and Parser: `Standard (Well) (;)`
- The dilution layout named `Dilution Factor 2 - 96` must exist in the system.

![Plate Serial Dilution Layout](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Setting/sample-jobs-refdata-dilution-layout-2.png?sv=2026-02-06&spr=https&st=2026-11-11T15%3A47%3A55Z&se=2026-11-11T16%3A47%3A55Z&sr=c&sp=r&sig=he%2B8j5dTjsEeMBLRTmFfSOb76NdKunqzf%2FODWKxmYA0%3D)

These should exist by default in the Sample application.

### Items

This tutorial requires specific plates as input. To create the required items, use the attached file [PS_Tutorial_Plates_Registration](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Documentation/discngine-assay-ps-tutorial-plates-registration.txt?sv=2026-02-06&spr=https&st=2026-11-11T15%3A47%3A55Z&se=2026-11-11T16%3A47%3A55Z&sr=c&sp=r&sig=he%2B8j5dTjsEeMBLRTmFfSOb76NdKunqzf%2FODWKxmYA0%3D) to process a Plates Registration from File workflow and a Container Reception workflow using external barcodes.

For more information about registration and reception, see the [Plates Registration from File tutorial](/public/docs/tutorial-registration-from-file).

## Workflow

### Job Initialization

[Create a new job](/public/docs/jobs-overview#create-a-job) and select the `Plate Solubilization` workflow.

### Requesting

- Select **Greiner PP384V** as the Target Plate Type
- Specify Request from **Text**
- Copy/paste the plate barcodes in the text area: **PS_DRY_PLATE_01:PS_DRY_PLATE_02**
- Click `Add IDs` to confirm. The plates are listed in the added requests.

              
              

The two plates used as request items come from the registration of the file "PS_Tutorial_Plates_Registration.txt". The batches in those plates doesn’t have a Molecular Weight. The message displayed next to them is only a warning about limitations (unable to request a dilution based on a molar concentration) but does not prevent you from using those plates in the workflow. Liquid plates cannot be used in this workflow.

- Click `Next`

### Task Requesting

- Source Selection Mode = **All Sources**
- Dilution Mode = **Concentration**
- Target Concentration = **100 mg/mL**
- Solvent = **DMSO**
- Click `Add Task`
- Click `Next`

### Sourcing

The Sourcing page is used to check that the requested operations are compatible with the source plates (information in the "Sourced" column) and available.

- Click `Next`

### Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

- Select the **Standard (Well) (;)** equipment
- Set File Order to **By Request** and Process Order to **By Column**
- Click `Generate worklist`

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing the information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

- Click `Copy Worklist`
- Click `Next` to propagate those changes in the system.  

The tasks are processed in the Sample application.

### Storing

For this workflow, there is no output container (transformations were directly applied to the source container).

- Do not change the location of the plates used; click `Next`.
- A message box asks if you want to increment the Thawing Cycle of the plates. Click `No` to continue.

### Pipe & Close

The [Pipe & Close step](/public/docs/jobs-overview#pipe-close) provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one. For this tutorial, click `Close & Quit` to finish the job.