---
title: "Tutorial - Plate Splitting"
slug: "tutorial-plate-splitting"
updated: 2026-09-09T14:03:40Z
published: 2026-09-09T14:03:40Z
canonical: "docs.discngine.com/tutorial-plate-splitting"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Tutorial - Plate Splitting

## Introduction

In this tutorial, you will learn how to split a plate into multiple smaller format plates using the Plate Splitting workflow.

## Prerequisite

              Required permissions and setup

              

**Sample Processing** role is required to access this workflow.

**Required system configuration:**

- Container Types: `Greiner PP96V`,
- Container Usage `Inventory`,
- Solvent `DMSO`,
- Worklist Generator and Parser: `Standard (Container) (;)`

Those should exist by default in Sample application.

### Items

Containers and Locations created from Discngine Sample’s automation will be used:

- 2 plates 384 (100 uL – 10 mM). **Layout with columns 1 and 24 as empty.**
- `Paris` location.

Automation is run during installation if “example_dataset” is set to true in the configuration file.

## Workflow

### Job Initialization

[Create a new job](/public/docs/jobs-overview#create-a-job) and select the `Plate Splitting` workflow.

### Requesting

- Set Source Type as **Plate**
- Set Target Plate Barcode as **Generate New Barcode**
- Select **Greiner PP96V** as the Target Plate Type
- Specify Request from **Text**
- Copy/paste the plate barcodes in the text area: **PTEST007:PTEST008**
- Click `Add IDs` to confirm. The plates are listed in the added requests.
- Click `Next`

### Task Requesting

- Source Selection Mode = **All Sources**
- Source Quadrant = **1**
- Target Usage = **Inventory**
- Number of Copy = **1**
- Target Volume = **30 μl**
- Dilution = **Target**
- Dilution Mode = **Dilution Factor**
- Dilution Factor = **10**
- Solvent = **DMSO**
- Offset Volume = **0%**
- Offset Solvent = **0%**
- Click `Add Task`. One task is added.  

**Repeat these steps for Quadrants 2, 3, and 4 (four tasks are created).**
- Click `Next`

### Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source plates (information in the "Sourced" column).

- Click `Next`

### Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

- Select the **Standard (Container) (;)** equipment
- Set File Order to **By Request**
- Click `Generate worklist`

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing the information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

- Click `Copy Worklist`
- Click `Next` to propagate those changes in the system.  

The tasks are processed in the Sample application.

### Storing

The [Storing step](/public/docs/jobs-overview#storing) allows you to assign a location to the items involved in the job. First, for the output plates (plates created during the job). Eight target 96-well plates have been created from the two input 384-well plates.

- Click `Assign Location to Selected`
- Select **Paris** and click `Select` to confirm your choice.
- Click `Next` to continue to the input plates. Here, we won’t change the input plates location.
- Click `Next`  

You can increment the Thawing Cycle count of the input plates if necessary.

### Pipe & Close

The [Pipe & Close step](/public/docs/jobs-overview#pipe-close) provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one. For this tutorial, click `Close & Quit` to finish the job.