Tutorial - Rack Creation from Plate

Introduction

In this tutorial, you will be guided through creating a 96-well tube rack from a 96-well plate by transferring its content. You can also create a rack from a different plate format, such as 384 wells, or create several racks at once.

Prerequisites

Required permissions and setup

Sample Processing role is required to access this workflow.

Required system configuration:

Plate Type: Greiner PP96V,
Container Usage Inventory,
Solvent DMSO,
Filling Layout: MIN(1) - MAX(12) (column filling),

Filling Layout

Worklist Generator and Parser: Standard (Container) (;)
Location Type: Rack with location mask(s): 96 and 384 (if you want to create a 384-well tube rack)

These should exist by default in the Sample application.

Items

Containers and Locations created from Discngine Sample’s automation will be used:

Plate PTEST001.
Location HTS Lab
Automation is running during installation if “example_dataset” is set to true in the configuration file.

Workflow

Job Initialization

Create a new job and select the Rack Creation from Plate workflow.

Requesting

Choose Plate as Source Type
Select Target Rack Barcode as Generate New Barcode
Select 96 (8x12) as the Target Rack Type.
Specify Request from Text
Paste the specific plate barcode in the Source area: PTEST001
Plate names can be colon- or line break-separated if you have several plates
Click Add IDs to confirm. The plate is listed in the added requests
Click Next.

Task Requesting

Choose Source Selection Mode: All sources
Select Target Usage: Inventory
Select Number of Copy: 1
Target Volume: 10 μl
Dilution: Target
Dilution Mode: Dilution Factor
Dilution Factor: 10
Buffer (Solvent): DMSO
Offset Volume: 0 %
Offset Solvent: 0 %
Click Add Task. One task is added.
Click Next.

Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source batches (see the Sourced column).

Click Next.

Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

Select the Standard (Container) (;) equipment and File Order: By Request
Click Generate worklist.

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute.
The Worklist Parser area expects a file containing information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

Click Copy Worklist.
Click Next to propagate these changes in the system.

The tasks are processed in the Sample application.

Storing

The Storing step allows you to assign a location to the items involved in the job.
First, for the output rack (the rack created during the job):

Click Assign Location to Selected.
Select Paris > HTS Lab > Freezer1 and click Select to confirm your choice.
Click Next to continue to the input plates with updated volume. Here, we won’t change the input plates' location.
Click Next.

You can increment the Thawing Cycle count of the input plates if necessary.

Pipe & Close

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow.
You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one.

For this tutorial, click Close & Quit to finish the job.