---
title: "Tutorial - Rack Replication"
slug: "tutorial-rack-replication"
updated: 2026-09-09T14:04:00Z
published: 2026-09-09T14:04:00Z
canonical: "docs.discngine.com/tutorial-rack-replication"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://docs.discngine.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Tutorial - Rack Replication

## Introduction

In this tutorial, you will create two copies of five 96-vial racks (100 μL – 10 mM). The resulting vials will each have a volume of 30 μL and will be diluted by a factor of 10 (resulting in 30 μL – 1 mM).

![Example of rack replication workflow](https://cdn.document360.io/dde9293e-c107-4b3e-9774-9c9c296ed970/Images/Setting/sample-rack-replication-example.png?sv=2026-02-06&spr=https&st=2026-11-11T19%3A35%3A34Z&se=2026-11-11T20%3A35%3A34Z&sr=c&sp=r&sig=8jRUyYpysp4515hjHVb7wNEKnJTaFlnLLGi7nBd7u8Q%3D)

## Prerequisites

              Required permissions and setup

              

**Sample Processing** role is required to access this workflow.

**Required system configuration:**

- Location Type `Rack` with Rack Mask(s): 96 and 384 (if you want to create a 384 well tube rack),
- Container Usage `Inventory`,
- Solvent `DMSO`,
- Worklist Generator and Parser: `Standard (Container) (;)`

These should exist by default in the Sample application.

### Items

Racks from Discngine Sample’s automation will be used:

- 5 racks 96 (100 uL – 10 mM). Layout with columns 1 and 12 as empty,
- Location `Paris > HTS Lab > Freezer1`  

Automation is running during installation if “example_dataset” is set to true in the configuration file.

## Workflow

### Job Initialization

[Create a new job](/public/docs/jobs-overview#create-a-job) and select the `Rack Replication` workflow.

### Requesting

- Select **Rack** as Source Type
- Choose Target Rack Barcode as **Generate New Barcode**
- Select **96 (8x12)** as the Target Rack Type
- Specify Request from **Text**
- Copy and paste the rack names in the text area: **R00020:R00019:R00018:R00017:R00016**  

Rack names can be separated by colons or line breaks.
- Click `Add IDs` to confirm. The racks are listed in the added requests.
- Click `Next`.

### Task Requesting

- Choose Source Selection Mode: **All sources**
- Target Usage: **Inventory**
- Select Number of Copy: **2**
- Target Volume: **30 μL**
- Dilution: **Target**
- Dilution Mode: **Dilution Factor**
- Dilution Factor: **10**
- Buffer (Solvent): **DMSO**
- Offset Volume: **0 %**
- Offset Solvent: **0 %**
- Click `Add Task`. Two tasks are added (one for each copy).
- Click `Next`.

### Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source racks (see the `Sourced` column).

- Click `Next`.

### Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

- Select the **Standard (Container) (;)** equipment and set File Order to **By Request**.
- Click `Generate worklist`.

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

- Click `Copy Worklist`.
- Click `Next` to propagate these changes in the system.

The tasks are processed in the Sample application.

### Storing

The [Storing step](/public/docs/jobs-overview#storing) allows you to assign a location to the items involved in the job. First, for the output racks (racks created during the job):

- Click `Assign Location to Selected`.
- Select **Paris > HTS Lab > Freezer1** and click `Select` to confirm your choice.
- Click `Next` to continue to the input racks. Here, we won’t change the input racks' location.
- Click `Next`.

You can increment the Thawing Cycle count of the input racks if necessary.

### Pipe & Close

The [Pipe & Close step](/public/docs/jobs-overview#pipe-close) provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input racks from this one.

For this tutorial, click `Close & Quit` to finish the job.